Unlike M100 (which provides “S/I/R” breakpoints for many drugs), M45 often provides or “provisional breakpoints” due to limited data. Labs must report results with interpretive comments.
The CLSI M45 document is an indispensable tool for microbiology laboratories tackling fastidious bacteria. By providing tailored testing methodologies, M45 bridges the gap between routine susceptibility testing and the needs of complex clinical cases, ensuring reliable, accurate, and actionable results for improved patient management.
Implementing M45 requires strict adherence to quality management systems. Laboratories should follow these steps when evaluating rare isolates: clsi document m45 pdf
The CLSI M45 document is protected by copyright and must be purchased directly from the Clinical and Laboratory Standards Institute.
Purchase options include both hard copy and electronic formats (PDF) from the CLSI shop. Unlike M100 (which provides “S/I/R” breakpoints for many
: Some online vendors also sell the CLSI M45 PDF. It's important to ensure these are legitimate to avoid using outdated or unauthorized copies that may not be compliant for regulatory purposes.
Disclaimer: This article is for informational purposes. Always refer to the latest, official CLSI documents for clinical laboratory practices. By providing tailored testing methodologies, M45 bridges the
The most recent and widely used version is the , published on August 15, 2016 by CLSI. Its scope is broad, covering a diverse list of bacterial genera and species that had previously lacked standardized methods. This includes organisms like:
Common environmental contaminants that can cause severe infections in immunocompromised patients.
Standard bacteria incubate at 35°C in ambient air. However, M45 specifies:
: Aeromonas spp., Vibrio spp., and Corynebacterium species.